@article {KNZ001277, title = {Massively parallel 454-sequencing of Quercus macrocarpa phyllosphere fungal communities indicates reduced richness and diversity in urban environments}, journal = {New Phytologist}, volume = {184}, year = {2009}, pages = {438 -448}, abstract = {

\•\ This study targeted the fungal communities in the phyllosphere of Quercus macrocarpa and compared the fungal species richness, diversity and community composition among trees located within and outside a small urban center using recently developed 454 sequencing and DNA tagging. \•\ The results indicate that the fungal phyllosphere communities are extremely diverse and strongly dominated by ascomycetes, with Microsphaeropsis [two Operational Taxonomic Units (OTUs); 23.6\%], Alternaria (six OTUs; 16.1\%), Epicoccum (one OTU; 6.0\%) and Erysiphe (two OTUs; 5.9\%) as the most abundant genera. \•\ Although the sequencing effort averaged 1000 reads per tree and detected nearly 700 distinct molecular OTUs at 95\% internal transcribed spacer 1 similarity, the richness of the hyperdiverse phyllosphere communities could not be reliably estimated as nearly one-half of the molecular OTUs were singletons. \•\ The fungal communities within and outside the urban center differed in richness and diversity, which were lower within the urban development. The two land-use types contained communities that were distinct and more than 10\% of the molecular OTUs differed in their frequency.

}, keywords = {LTER-KNZ}, doi = {10.1111/j.1469-8137.2009.02990.x}, url = {https://nph.onlinelibrary.wiley.com/doi/full/10.1111/j.1469-8137.2009.02990.x}, author = {A. Jumpponen and Jones, K.L.} }