@article {KNZ001101, title = {Development of taxon-specific markers for high-throughput screening of microbial-feeding nematodes}, journal = {Molecular Ecology Notes}, volume = {6}, year = {2006}, pages = {712 -714}, abstract = {In an effort to assess the taxonomic identity of large-scale samplings of nematodes from the Konza Tallgrass Prairie, we sequenced a portion of the 18S rRNA gene and its associated internally transcribed spacer (ITS1) from 74 nematodes encompassing four taxonomic families. From these sequences, we have developed a series of molecular probes to distinguish 16 distinct microbivorous nematode taxa. Using a combination of low power microscopy and taxon-specific real-time probes, the 74 nematodes were correctly assigned to their respective taxonomic groups. This optimized method provides a high-throughput assay to determine nematode identities across larger data sets.}, keywords = {LTER-KNZ, 18S rRNA, 5' nuclease assaym, Nematoda, real-time PCR, Taqman}, doi = {10.1111/j.1471-8286.2006.01319.x}, author = {Jones, K.L. and Todd, T.C. and Herman, M.A.} }