@phdthesis {KNZ001835, title = {Patterns and ecological consequences of water uptake, redistribution, and loss in tallgrass prairie}, volume = {PhD Dissertation}, year = {2016}, school = {Kansas State University}, type = {Ph.D. Thesis}, address = {Manhattan, KS}, abstract = {

Water availability is a key driver of many plant and ecosystem processes in tallgrass prairies, yet we have a limited understanding of how grassland plants utilize water through space and time. Considering that tallgrass prairies experience tremendous heterogeneity in soil resources, identifying spatiotemporal variation in plant ecohydrology is critical for understanding current drivers of plant responses to water and for predicting ecosystem responses to future changes in climate. Here, I investigated the patterns, drivers, and ecological consequences of plant water use (e.g., water uptake, water redistribution, and water loss) in a native tallgrass prairie located in northeastern Kansas, USA. Using a combination of leaf gas exchange, sap flow, and isotopic techniques, I addressed four main questions: 1) How does fire and grazing by bison impact use of water from different sources and niche overlap for common grasses, forbs, and shrubs? 2) Does hydraulic lift occur in grazed and ungrazed tallgrass prairie, and does this impact facilitation for water within grassland communities? 3) What are the patterns and drivers of nocturnal transpiration in common grassland species? 4) How does diel stem sap flow and canopy transpiration vary among common grassland species?

I found that bison grazing increased the depth of water uptake by Andropogon gerardii and Rhus glabra, reducing niche overlap with co-occurring species. Conversely, grazing did not affect hydraulic lift, which was generally uncommon and likely limited by nocturnal transpiration. Further, leaf gas exchange measurements indicated that nocturnal transpiration occurred commonly in tallgrass prairie plants and was greatest among grasses and early in the growing season. Nocturnal transpiration was not driven by vapor pressure deficit or soil moisture, as commonly observed in other systems, but was regulated by nocturnal stomatal conductance in most species. Finally, I found that daytime sap flow rates were variable among species and functional types, with larger flux rates among woody species. Nocturnal sap flow rates were more consistent across species, which caused nighttime sap flow and transpiration to account for a larger proportion of daily flux rates in grasses than in forbs or shrubs. These results show that water uptake, water redistribution, and water loss are all influenced by different biotic and abiotic drivers and have varying ecological impacts across a heterogeneous landscape. Additionally, extensive differences in water flux exist among co-occurring species and plant functional groups, which likely reflect varying strategies to tolerate water limitation. These results suggest that shifts in the abundance of these species with future climate changes, or with ecosystem state changes, will likely impact ecosystem-level water balance.

}, keywords = {LTER-KNZ, Grasslands, Isotopes, Plant physiology, Sap flow, Transpiration}, url = {http://krex.k-state.edu/dspace/handle/2097/34514}, author = {O{\textquoteright}Keefe, Kimberly} } @article {KNZ001606, title = {Evidence of physiological decoupling from grassland ecosystem drivers by an encroaching woody shrub}, journal = {PLoS ONE}, volume = {8}, year = {2013}, pages = {81630 -}, abstract = {

Shrub encroachment of grasslands is a transformative ecological process by which native woody species increase in cover and frequency and replace the herbaceous community. Mechanisms of encroachment are typically assessed using temporal data or experimental manipulations, with few large spatial assessments of shrub physiology. In a mesic grassland in North America, we measured inter- and intra-annual variability in leaf δ13C in Cornus drummondii across a grassland landscape with varying fire frequency, presence of large grazers and topographic variability. This assessment of changes in individual shrub physiology is the largest spatial and temporal assessment recorded to date. Despite a doubling of annual rainfall (in 2008 versus 2011), leaf δ13C was statistically similar among and within years from 2008-11 (range of \−28 to \−27\‰). A topography*grazing interaction was present, with higher leaf δ13C in locations that typically have more bare soil and higher sensible heat in the growing season (upland topographic positions and grazed grasslands). Leaf δ13C from slopes varied among grazing contrasts, with upland and slope leaf δ13C more similar in ungrazed locations, while slopes and lowlands were more similar in grazed locations. In 2011, canopy greenness (normalized difference vegetation index \– NDVI) was assessed at the centroid of individual shrubs using high-resolution hyperspectral imagery. Canopy greenness was highest mid-summer, likely reflecting temporal periods when C assimilation rates were highest. Similar to patterns seen in leaf δ13C, NDVI was highest in locations that typically experience lowest sensible heat (lowlands and ungrazed). The ability of Cornus drummondii to decouple leaf physiological responses from climate variability and fire frequency is a likely contributor to the increase in cover and frequency of this shrub species in mesic grassland and may be generalizable to other grasslands undergoing woody encroachment.

}, keywords = {LTER-KNZ, Ecosystems, Grasslands, Grazing, Leaves, Plant physiology, Seasons, Shrubs, Water resources}, doi = {10.1371/journal.pone.0081630}, url = {https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0081630}, author = {Jesse B. Nippert and Ocheltree, T.W. and Orozco, G.L. and Z. Ratajczak and Ling, B. and Skibbe, A.M.} }