01579nas a2200157 4500008004100000245009000041210006900131300001200200490000700212520108000219100001601299700001601315700001501331700001701346856005801363 2012 eng d00aSeveral grassland soil nematodes species are insensitive to RNA-mediated interference0 aSeveral grassland soil nematodes species are insensitive to RNAm a92 -1010 v443 a
Phenotypic analysis of defects caused by RNA mediated interference (RNAi) in Caenorhabditis elegans has proven to be a powerful tool for determining gene function. In this study we investigated the effectiveness of RNAi in four non-model grassland soil nematodes, Oscheius sp FVV-2., Rhabditis sp, Mesorhabditis sp., and Acrobeloides sp. In contrast to reference experiments performed using C. elegans and Caenorhabditis briggsae, feeding bacteria expressing dsRNA and injecting dsRNA into the gonad did not produce the expected RNAi knockdown phenotypes in any of the grassland nematodes. Quantitative reverse-transcribed PCR (qRT-PCR) assays did not detect a statistically significant reduction in the mRNA levels of endogenous genes targeted by RNAi in Oscheius sp., and Mesorhabditis sp. From these studies we conclude that due to low effectiveness and inconsistent reproducibility, RNAi knockdown phenotypes in non-Caenorhabditis nematodes should be interpreted cautiously.
1 aWheeler, D.1 aDarby, B.J.1 aTodd, T.C.1 aHerman, M.A. uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3593264/