01387nas a2200145 4500008004100000245008800041210006900129300001300198490000800211520092500219100001701144700001601161700001601177856004801193 2011 eng d00aAssessing fine-scale genotypic structure of a dominant species in native grasslands0 aAssessing finescale genotypic structure of a dominant species in a211 -2240 v1653 a
Genotypic diversity of dominant species has been shown to have important consequences for community and ecosystem processes at a fine spatial scale. We examined the fine-scale (i.e., plant neighborhood scale, <1 m2) genotypic structure of Andropogon gerardii, a dominant species in the tallgrass prairie, which is a productive and endangered grassland ecosystem, employing the commonly used amplified fragment length polymorphism (AFLP) technique. In this paper we used two methods to assess the fine-scale genetic spatial structure of a dominant perennial grass, (1) we determined how many tillers to sample in a 1 m2 area and (2) we developed AFLP markers that would differentiate between genotypes. By determining appropriate sampling and molecular techniques, our findings can be applied to questions addressing how genetic diversity of dominant species affect ecosystem processes in the tallgrass prairie.
1 aAvolio, M.L.1 aChang, C.C.1 aSmith, M.D. uhttps://doi.org/10.1674/0003-0031-165.2.211