We conducted a series of stepwise NO3− additions to investigate the response of NO3− uptake to short-term (acute) changes in N concentration in 3 prairie streams. Observed NO3− uptake rates increased with short-term elevations in NO3− concentration and were consistent with linear and Michaelis–Menten kinetics models. We compiled these data with uptake rates from additional published studies to calculate robust estimates of N uptake kinetics for prairie streams. Half-saturation coefficients based on compiled data were 6.7 µg/L for NH4+ and 67 µg/L for NO3−-N. This difference in half-saturation coefficients suggests that NH4+ is more efficiently assimilated than NO3−, indicating a preference for NH4+ as an N source. Similarly, ambient concentrations of NH4+ and NO3− were less than their respective half-saturation coefficients, and aerial uptake rates were generally <5% of the maximum, suggesting severe limitation of N uptake at ambient conditions. The observed pattern of uptake kinetics suggests that physiological constraints limit biotic N uptake in these low-N streams and contrasts with the pattern of uptake observed in streams with chronically elevated ambient NO3− concentrations.

10aBiogeochemistry10aKings Creek10aKonza Prairie10anitrate uptake1 aO'Brien, J.M.1 aDodds, W., K. uhttps://www.journals.uchicago.edu/doi/10.1899/09-021.1