TY - JOUR T1 - Development of taxon-specific markers for high-throughput screening of microbial-feeding nematodes JF - Molecular Ecology Notes Y1 - 2006 A1 - Jones, K.L. A1 - Todd, T.C. A1 - Herman, M.A. KW - 18S rRNA KW - 5′ nuclease assaym KW - Nematoda KW - real-time PCR KW - Taqman AB - In an effort to assess the taxonomic identity of large-scale samplings of nematodes from the Konza Tallgrass Prairie, we sequenced a portion of the 18S rRNA gene and its associated internally transcribed spacer (ITS1) from 74 nematodes encompassing four taxonomic families. From these sequences, we have developed a series of molecular probes to distinguish 16 distinct microbivorous nematode taxa. Using a combination of low power microscopy and taxon-specific real-time probes, the 74 nematodes were correctly assigned to their respective taxonomic groups. This optimized method provides a high-throughput assay to determine nematode identities across larger data sets. VL - 6 ER -