Title | Development of taxon-specific markers for high-throughput screening of microbial-feeding nematodes |
Publication Type | Journal Article |
Year of Publication | 2006 |
Authors | Jones, KL, Todd, TC, Herman, MA |
Journal | Molecular Ecology Notes |
Volume | 6 |
Pagination | 712 -714 |
Accession Number | KNZ001101 |
Keywords | 18S rRNA, 5′ nuclease assaym, Nematoda, real-time PCR, Taqman |
Abstract | In an effort to assess the taxonomic identity of large-scale samplings of nematodes from the Konza Tallgrass Prairie, we sequenced a portion of the 18S rRNA gene and its associated internally transcribed spacer (ITS1) from 74 nematodes encompassing four taxonomic families. From these sequences, we have developed a series of molecular probes to distinguish 16 distinct microbivorous nematode taxa. Using a combination of low power microscopy and taxon-specific real-time probes, the 74 nematodes were correctly assigned to their respective taxonomic groups. This optimized method provides a high-throughput assay to determine nematode identities across larger data sets. |
DOI | 10.1111/j.1471-8286.2006.01319.x |