|Title||Development of taxon-specific markers for high-throughput screening of microbial-feeding nematodes|
|Publication Type||Journal Article|
|Year of Publication||2006|
|Authors||Jones, KL, Todd, TC, Herman, MA|
|Journal||Molecular Ecology Notes|
|Keywords||18S rRNA, 5′ nuclease assaym, Nematoda, real-time PCR, Taqman|
In an effort to assess the taxonomic identity of large-scale samplings of nematodes from the Konza Tallgrass Prairie, we sequenced a portion of the 18S rRNA gene and its associated internally transcribed spacer (ITS1) from 74 nematodes encompassing four taxonomic families. From these sequences, we have developed a series of molecular probes to distinguish 16 distinct microbivorous nematode taxa. Using a combination of low power microscopy and taxon-specific real-time probes, the 74 nematodes were correctly assigned to their respective taxonomic groups. This optimized method provides a high-throughput assay to determine nematode identities across larger data sets.