CMY01 English utf8 dataset Konza LTER 785-532-6729 Division of Biology Manhattan KS 66506 US knzlter@ksu.edu http://lter.konza.ksu.edu pointOfContact 2015-06-15 ISO 19115-2 Geographic Information - North American Profile Metadata - Data with Biological Extensions ISO 19115-2:2009(E) CMY01 Mycorrhizal colonization and plant community responses to long-term suppression of Mycorrhizal Fungi 2015-06-15 publication Gail Wilson 405-744-5539 008C Ag Hall, Natural Ressource Ecology & Management, Oklahoma State University Stillwater OK 74077 US gail.wilson@okstate.edu pointOfContact documentDigital Twenty replicate permanent 2x2 m plots were established in early 1991 along a randomly located transect, with a 2m space between each plot, on the following watersheds: 1B, 1D, annually burned HQB, 10B, 20D and infrequently burned HQB. Ten of the plots were randomly assigned as long-term mycorrhizal suppression plots. In each of these plots, AM fungi were suppressed by the application of the fungicide benomyl as a soil drench (7.5 liters per plot) at the rate of 1.25 g/m2 (active ingredient). The mycorrhizal suppression plots were treated biweekly throughout each growing season (April through October) beginning in 1991. The control plots each received no fungicide, but an equivalent volume of water (7.5 liters) was applied biweekly. To evaluate the effectiveness of the fungicide, three soil cores (2.5 cm diameter x 14 cm deep) were removed from both fungicide-treated and control plots each October throughout the study. Roots were extracted from the soil, washed free of soil, stained in trypan blue (Phillips and Hayman, 1970), and examined microscopically to assess percentage root colonization by mycorrhizal fungi using a Petri dish scored in 1-cm squares (Daniels et al.1981). Study plant community and mycorrhizal colonization response to long-term suppression of mycorrhizal fungi. completed Konza LTER 785-532-6729 Division of Biology Manhattan KS 66506 US knzlter@ksu.edu http://lter.konza.ksu.edu pointOfContact unknown complete Primary Production Populations theme Core Areas English watershed 1B -96.5942 -96.603 39.0666 39.0736 1D: Ungrazed and scheduled prescribed burned annually in the spring. -96.5594 -96.5658 39.0732 39.0841 HQB : Ungrazed and scheduled prescribed burned annually in the spring -96.6076 -96.6128 39.101 39.1066 SpA: Ungrazed and scheduled prescribed burned annually in the spring. -96.5881 -96.5956 39.0683 39.0784 R1B: Ungrazed and scheduled unburned through 2000, scheduled prescribed burned annually in the spring beginning 2001 -96.5481 -96.5579 39.0731 39.0792 ground condition 1991-04-01 1995-10-31 http://lter.konza.ksu.edu/sites/default/files/data/CMY011.csv CMY011 eng; US Konza Prairie LTER CMY011 Data Source Definition : Mycorrhizal Suppression Plots Record Delimiter : \r\n Number of Header Lines : 1 Orientation : Column Quote Character : "Field Delimiter : , false rowid rowid in file (internal book keeping) DataCode Dataset code RecType Record type RecYear Year of record RecMonth Month of record Date Time Format: MM RecDay Day of record Date Time Format: DD Transect transect code a HQB b HQB c 1B d 10B e 1D f 20D Burn burn frequency b annual u infrequent Plot Plot number Fungicide fungicide usage ben fungicide myc control Species species abbreviation code Form growth form Sample repeated measure 1-4 Vegetative number of vegetative pin contacts Reproductive number of reproductive pin contacts DBF http://lter.konza.ksu.edu/sites/default/files/data/CMY011.csv dataset Location of Sampling Stations: Watersheds 1B, 1D, annual burned HQB, 10B, 20D and infrequently burned HQB. Frequency of Sampling: To evaluate the effectiveness of the fungicide, three soil cores were removed from both fungicide-treated and control plots each October. Methods: Twenty replicate permanent 2x2 m plots were established in early 1991 along a randomly located transect, with a 2m space between each plot, on the following watersheds:1B,1D, annually burned HQB,10B, 20D and infrequently burned HQB. Ten of the plots were randomly assigned as long-term mycorrhizal suppression plots. In each of these plots, AM fungi were suppressed by the application of the fungicide benomyl as a soil drench (7.5 liters per plot) at the rate of 1.25 g/m2 (active ingredient). The mycorrhizal suppression plots were treated biweekly throughout each growing season (April through October) beginning in 1991. The control plots each received no fungicide, but an equivalent volume of water (7.5 liters) was applied biweekly. To evaluate the effectiveness of the fungicide, three soil cores (2.5 cm diameter x 14 cm deep) were removed from both fungicide-treated and control plots each October throughout the study. Roots were extracted from the soil, washed free of soil, stained in trypan blue (Phillips and Hayman, 1970), and examined microscopically to assess percentage root colonization by mycorrhizal fungi using a Petri dish scored in 1-cm squares (Daniels et al. 1981). For additional metadata information see: http://lter.konza.ksu.edu/sites/default/files/DC.pdf For additional methods information see: http://lter.konza.ksu.edu/sites/default/files/MM.pdf Metadata Access Constraints: none Metadata Use Constraints: none annually Konza LTER http://www.konza.ksu.edu/ pointOfContact