The concentration and isotopic composition of soil carbon and nitrogen were measured from select archived soil cores originally collected for the NSC01 dataset using an isotope ratio mass spectrometer coupled with an elemental analyzer. These soil cores were collected from the lowlands (25 cm depth) of four experimental watersheds in 1982, 1987, 2002, 2010, and 2015. The four experimental watersheds are 001d, n01b, 020b, and n20b.