We collected Carabidae, Gryllidae, and Acrididae from three areas in two locations in Kansas to determine if their nutrient and energy characteristics were reated to soil fertility. Carbon content (%) of Acrididae was greater in samples collected on areas with higher soil carbon and nitrogen. Protein content (%) of Carabidae biomass was not related to soil fertility whereas Acrididae was. Calcium and phosphorus content (ppm) of Carabidae biomass did not differ by area, but potassium connntent did. Calcium, phosphorus, potassium, and energy (cal/g) content of Gryllidae biomass varied with frequency of burning in tallgrass prairie. Energy content of Carabidae biomass varied by area and burning; frequency. Protein content did not differ among the three invertebrate taxa, but calcium, phosphorus, and potassium content did. Energy content of Carabidae was greater than that of Acrididae. Differences in nutrient and energy content of invertebrate biomass appeared to be related to vegetation on the areas, which was a reflection of soil fertility.